Laszlo Nanassy

László Nánássy, Ph.D.
Current Employment:
Laboratory Director at Versys Clinics Human Reproduction Institute, Budapest, Hungary
Education:
Senior Clinical Embryologist ESHRE certificate 2013
Registered Clinical Scientist with the Health Professions Council (UK) – 2011-2013
Post-Doctoral Fellowship at the University of Utah School of Medicine, Andrology and IVF Laboratories, Salt Lake City, Utah, USA, 2007-2010.
Research: Epimutations in sperm of infertile males
Ph.D. in Animal Scienceat the University of Debrecen, Center of Agricultural Sciences, Department of Animal Science, Debrecen, Hungary, 2003-2007.
Research performed at Purdue University, Department of Animal Sciences, Indiana, USA
Thesis: Evaluation of the production of parthenogenetic porcine embryos.
Master of Science in Animal Science at the University of West Hungary Faculty of Agricultural and Food Sciences, Mosonmagyarovar, Hungary, 2003.
Thesis: Sperm evaluation by Micro Swim Up method.
Professional Experience:
2012- Laboratory Director at Versys Clinics Human Reproduction Institute, Budapest, Hungary. Duties: day-to-day operation of embryology and andrology laboratories, oocyte retrieval, IVF/ICSI insemination, embryo grading, embryo transfer, assisted hatching, blastomere biopsy, blastomere fixation, embryo cryopreservation, general laboratory maintenance, QA/QC, laboratory statistics
2011- 2012; Senior Embryologist at The Fertility Institutes, Los Angeles, California, USA. Duties: oocyte retrieval, IVF/ICSI insemination, embryo grading, embryo transfer, assisted hatching, blastomere biopsy, blastomere fixation, embryo cryopreservation, general laboratory maintenance, QA/QC, laboratory statistics
2011-2011; Clinical Embryologist at Galway Fertility, Galway, Ireland. Duties: daily andrology (semen analysis, sperm preparation, sperm freezing) and embryology (oocyte retrieval, IVF/ICSI insemination, embryo grading, embryo transfer, assisted hatching, embryo cryopreservation, general laboratory maintenance) work, QA/QC
2007-2010; Post-Doctoral Fellow at the University of Utah School of Medicine, Andology and IVF Laboratories, Salt Lake City, Utah, USA. Research projects: gene expression in preimplantation embryos, culture conditions during early embryo development, epigenetic background of male infertility. Clinical duties: andrology and embryology cover.
2003-2007; Graduate student at University of Debrecen Center of Agricultural Sciences, Department of Animal Science, Hungary, 2003-2007.
2005-2006; Research Scholar at Purdue University, Department of Animal Science, West Lafayette, Indiana, USA. Research projects: investigation of the efficiency of parthenogenetic activation methods of porcine oocytes, nuclear transfer in pig; defining culture conditions for preimplantation porcine embryos; evaluation of MPF and MAP kinase dynamics following artificial activation of pig oocytes.
2002-2003; Research worker-undergraduate student, Agricultural Biotechnology Center,
Laboratory of Embryology, Animal Reproduction Group. Research activities: evaluation of bovine sperm preparation techniques, in vitro embryo production.
Training courses:
  • Polar body, cleavage and tropectoderm biopsy for Preimplantation Genetic Diagnosis Workshop at University College London 2013 (Dr. Joyce Harper)
  • Cryopreservation and Transplantation of Human Ovarian Tissue and Preantral Follicle Isolation and In Vitro Culture 2013 at Université catholique de Louvain (Prof. Dr. Marie-Madeleine Dolmans)
  • Application and challenges of emerging technologies in preimplantation and prenatal diagnosis ESHRE workshop 2013
  • Cryotop Hands-on Vitrification Workshop (Kitazato-Dibimed) (Dr. Ana Cobo)
ART Related Skills:
Semen analysis, sperm preparation techniques (sperm wash, density gradient and swim-up), anti-sperm antibody test, oocyte retrieval, microdrop insemination, ICSI, IMSI, embryo transfer, conventional gamete cryopreservation, vitrification (cryotip, cryoloop, cryotop), assisted hatching (laser, tyrode, partial zona dissection), polar body/blastomere/tropedoderm biopsy, blastomere fixation for FISH, blastomere/trophoblast fixation for PCR/microarray-based methods, QA/QC
Research Skills:
In vitro techniques: oocyte maturation, oocyte activation, somatic cell nuclear transfer (oocyte enucleation and donor cell injection), TUNEL assay, protein kinase assays, immunocytochemistry
Molecular biology techniques: DNA/RNA isolation, primer designing, probe designing, gene promoter analysis, PCR/multiplex PCR, RT-PCR, qPCR/Taqman, single cell gene expression analysis, Y chromosome microdeletion analysis, gene polymorphism analysis, DNA methylation analysis, bisulfite PCR design, pyrosequencing, amplicon sequencing, molecular cloning, transfection, vector handling, bacteria culture
Memberships in Professional Societies:
European Society of Human Reproduction and Embryology (ESHRE); 2011-
American Society for Reproductive Medicine (ASRM); 2008-
International Embryo Transfer Society (IETS); 2005-2007
Society for the Study of Reproduction (SSR); 2005-2007
Ad hoc reviewer:
Fertility and Sterility
Journal of Andrology
Other Skills:
Extensive knowledge of Windows and Mac operation systems and Microsoft Office programs (Word, Excel and PowerPoint)
Familiar with SPSS and STATA statistical programs
Awards:
Assistant Professor honoris causa at University of Debrecen, Debrecen, Hungary, 2011
Hungarian Eötvös Scholarship Foundation (Hungary) to Purdue University, 5 months, 2006
Széchenyi István Scholarship Foundation (Hungary) to Purdue University, 12 months, 2005
Participant in Hungarian National Scientific Student Conference 2003
First place at the Scientific Student Conference, University of West Hungary 2002
Peer-Reviewed Publications:
Nanassy L., Carrell DT. Abnormal methylation of the promoter of CREM is broadly associated with male infertility and poor semen quality, but is improved in sperm selected by density gradient centrifugation. Fertility and Sterility, 2011 95:2310-2314.
Nanassy L., Liu L., Griffin J., Carrell DT. The clinical utility of the protamine 1/protamine 2 ratio in sperm. Protein and Peptide Letters, 2011 18(8):772-777.
Nanassy L., Carrell DT. Analysis of the methylation pattern of six gene promoters in sperm of men with abnormal protamination.Asian Journal of Andrology, 2011 13:342-346.
Nanassy L., Griffin J., Emery BR., Carrell DT. The marmoset and cotton rat as animal models for the study of sperm chromatin packaging. Systems Biology in Reproductive Medicine, 2010 56(3):207-212.
Nanassy L., Peterson CA., Wilcox AL., Peterson CM., Hammoud A., Carrell DT. Comparison of 5% and ambient oxygen during days 3-5 of in vitro culture of human embryos. Fertility and Sterility, 2010 93(2):579-585.
Lee K., Hyslop JM., Nanassy L., Machaty Z. Incidence of apoptosis in parthenogenetic porcine embryos generated by using protein kinase sysnthesis inhibitors. Animal Reproduction Science, 2009 112: 261-272.
Nanassy L., Carrell DT. Paternal effects on early embryogenesis. Journal of Experimental & Clinical Assisted Reproduction, 2008 5:2.
Nánássy L., Lee K., Jávor A., Macháty Z. Changes in MPF and MAPK activities in porcine oocytes activated by different methods. Theriogenology, 2007 68:146-152.
Nánássy L., Lee K., Jávor A., Macháty Z. The effect of activation methods and culture conditions on the development of parthenogenetic porcine embryos. Animal Reproduction Science, 2007 104:264-274.
Zomborszky Z., Nagy S., Nánássy L., Szabari M., Bodo S. Experiences in deer sperm cryopreservation under practical conditions - A pilot study. Animal Reproduction Science, 2005 90:185-90.
Presentations (Society Meetings):
Oral presentation:
Nanassy L., Carrell DT. Semen characteristics of a large cohort show protamine 1 (P1) to protamine 2 (P2) ratio is independent of other sperm parameters. 66th Annual meeting of the American Society for Reproductive Medicine, Denver, USA; Fertility and Sterility, 2010 94(4):S61
Lee K., Nánássy L., Macháty Z. The distribution of STIM 1 in porcine oocytes and its potential role in capacitative Ca2+ entry. 39th Annual Meeting of the Society of Study for Reproduction; Biology of Reproduction, 2006 (Suppl. 1.)142.
Abstracts:
Nanassy L., Gajdocsi E., Nemes A., Antal F., Vereczkey A.No difference in embryo development and aneuploidy rates between successful and unsuccessful cycles following embryo transfer of euploid embryo(s) for patients with advanced maternal age. 2014 ESHRE Munich
Antal F., Teglas Gy., Bathori Gy., Csenki M., Debreceni D., Nanassy L., Schonleber J., Vereczkey A. Preimplantation genetic screening of embryos from couples experiencing repeated implantation failure. 2014 Reprod BioMed Online 28 S16
Nanassy L., Gajdocsi E., Nemes A., Antal F., Vereczkey A. No difference in clinical pregnancy and implantation rates between single and double transfer of euploid embryo(s). 2014 Reprod BioMed Online 28 S17
Nanassy L., Gajdocsi E., Nemes A., Antal F., Teglas G., Vereczkey A. Blastulation and pregnancy rates following cleavage stage biopsy in preimplantation genetic screening (PGS) cycles. 2014 PGDIS Conference Canterbury, UK
Nanassy L., Gajdocsi E., Dudas B., Antal F., Vereczkey A. Evaluation of in vitro fertilization combined with preimplantation genetic screening in a small setup. COGI Vienna 2013
Nanassy L., Dudas B., Kosa Z., Savay S., Debreceni D., Vereczkey A. Comparison of single and sequential culture systems on embryos development and aneuploidy rates using sibling oocytes; Fertility and Sterility, 2013 100(3):S247.
Vereczkey A., Kosa Z., Savay S., Csenki M., Nanassy L., Dudas B., Domotor Z., Debreceni D. Introducing preimplantation genetic screening by array comparative genomic hybridization in Hungary: improving outcome of in vitro fertilization; 2013 Human Reproduction 28:300.
Debreceni D., Csenki M., Kosa Z., Savay S., Nanassy L., Dudas B., Domotor Z., Vereczkey A. First preimplantation genetic diagnosis by microarray comparative genetic hybridization for Robertsonian translocation in Hungary – Case report; 2013 Reproductive BioMedicine Online 26(1):S47-48.
Nanassy L., Liu L., Griffin J., Carrell DT. Protamine 1 (P1) to protamine 2 (P2) ratio, sperm and hormonal parameters of a non-selected population. 4th Utah-Florence Symposium on the Genetics of Male Infertility 2010.
Nanassy L., Carrell DT. The methylation status of the promoter of the cAMP responsive element modulator (CREM) gene in patients with abnormally high or low protamine 1 (P1) to protamine 2 (P2) ratio. 4th Utah-Florence Symposium on the Genetics of Male Infertility 2010.
Nanassy L., Carrell DT. The methylation status of the promoters of OCT4, SOX2, HOXC11, MIR17 and NANOG genes in infertile men. 65th Annual meeting of the American Society for Reproductive Medicine; Fertility and Sterility, 2009 92(3):S211.
Nanassy L., Peterson CA., Aston KI., Peterson CM., Gibson M., Carrell DT. Reduced oxygen concentration during day 3-5 of embryo culture does not result in improved embryo quality. 64th Annual meeting of the American Society for Reproductive Medicine; Fertility and Sterility, 2008 90:S351.
Nanassy L., Peterson CA., Wilcox AL., Jones KP., Hatasaka HH., Carrell DT. Comparison of single and sequential culture systems on embryo development, pregnancy and implantation rates using sibling oocytes. 64th Annual meeting of the American Society for Reproductive Medicine; Fertility and Sterility, 2008 90:S431.
Nánássy L., Lee K., Jávor A., Macháty Z. Dynamics of mitogen-activated protein kinase activity in pig oocytes following parthenogenetic activation by different methods. 33rd Annual Conference of the International Embryo Transfer Society; Reproduction, Fertility and Development, 2007 19(1):282.
Nánássy L., Lee K., Jávor A., Cabot R., Macháty Z. Optimization of parthenogenetic oocyte activation for porcine nuclear transfer. 39th Annual Meeting of the Society of Study for Reproduction; Biology of Reproduction, 2006 (Suppl. 1.) 123.
Nánássy L., Lee K., Jávor A., Macháty Z. Changes in MPF activity in porcine oocytes following activation by various methods. 32nd Annual Conference of the International Embryo Transfer Society; Reproduction, Fertility and Development, 2006 18(1,2):265.
Nagy Sz., Szabari M., Nanassy L., Bodo Sz., Zomborszky Z. Fallow deer sperm cryopreservation – First experiences in Hungary. 9th Annual Conference of the European Society for Domestic Animal Reproduction (ESDAR); Reproduction in Domestic Animals, 2005 40(4):390
Nánássy L., Nagy Sz., Gócza E., Jávor A., Bodó Sz. Maximum yield of spermatozoa at 30 minutes in conventional and Micro-Swim Up. Joint European Conference in Reproduction and Fertility;Reproduction,2004 31:26.
Nagy E., Bali Papp A., Nanassy L., Ordog V., Nemenyi M., Dohy J., Ivancsics J. Evaluation by environmental scanning electron microscopy (ESEM) of pig oocytes matured and fertilized in vitro. Annual Conference of the International Embryo Transfer Society; Theriogenology, 2003 59:494.
Petz B., Bali Papp A., Somfai T., Nanassy L., Pecsi T., Ivancsics J. Effect of Percoll or short time centrifugation treatment on viability and acrosome integrity of frozen/thawed boar spermatozoa. Annual Conference of the International Embryo Transfer Society; Theriogenology, 2002 57:681.